Deducing Free-Energy Landscape of Biomolecular Dynamics from Cryo-Electron Microscopy Images
نویسندگان
چکیده
Cryo-electron microscopy（cryo-EM）is a powerful method to visualize high resolution structures of biomolecules under physiological condition. Since cryo-EM images represent conformational ensemble the target biomolecule, is applicable analyze biomolecular dynamics such as metastable conformations and/or free-energy landscape in changes. Here, we briefly introduce our deduce from and compare it with other existing methods.
منابع مشابه
Sinogram Denoising of Cryo-Electron Microscopy Images
Cryo-electron microscopy has recently been recognized as a useful alternative to obtain three-dimensional density maps of macromolecular complexes, especially when crystallography and NMR techniques fail. The three-dimensional model is constructed from large collections of cryo-electron microscopy images of identical particles in random (and unknown) orientations. The major problem with cryo-el...
متن کاملViewing Angle Classification of Cryo-Electron Microscopy Images Using Eigenvectors
The cryo-electron microscopy (cryo-EM) reconstruction problem is to find the three-dimensional structure of a macromolecule given noisy versions of its two-dimensional projection images at unknown random directions. We introduce a new algorithm for identifying noisy cryo-EM images of nearby viewing angles. This identification is an important first step in three-dimensional structure determinati...
متن کاملCohomology of Cryo-Electron Microscopy
The goal of cryo-electron microscopy (EM) is to reconstruct the 3-dimensional structure of a molecule from a collection of its 2-dimensional projected images. In this paper, we show that the basic premise of cryo-EM—patching together 2-dimensional projections to reconstruct a 3-dimensional object—is naturally one of Čech cohomology with SO(2)-coefficients. We deduce that every cryoEM reconstruc...
متن کاملDynamics of antibodies from cryo-electron tomography.
The issue of protein dynamics and its implications in the biological function of proteins are arousing greater and greater interest in molecular biology. In cryo-electron tomography experiments one takes several snapshots of a given biological macromolecule. In principle, a large enough collection of snapshots may then be used to calculate its equilibrium configuration in terms of the experimen...
متن کاملMathematics for cryo - electron microscopy
Single-particle cryo-electron microscopy (cryo-EM) has recently joined X-ray crystallography and NMR spectroscopy as a high-resolution structural method for biological macromolecules. Cryo-EM was selected by Nature Methods as Method of the Year 2015, large scale investments in cryo-EM facilities are being made all over the world, and the Nobel Prize in Chemistry 2017 was awarded to Jacques Dubo...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of the Crystallographic Society of Japan
سال: 2022
ISSN: ['0369-4585', '1884-5576']
DOI: https://doi.org/10.5940/jcrsj.64.300